DNA
Suffix

Part:BBa_G00001:Design

Designed by: Reshma Shetty   Group: BE.109 Instructors   (2006-03-13)

BioBrick cloning site suffix


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Please see Tom Knight's technical report for design notes on this part.

Source

References

  • T. F. Knight. [http://hdl.handle.net/1721.1/21168 Idempotent vector design for standard assembly of BioBricks]. Technical report, MIT DSpace archive, 2003.